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FruitBreedomics MAB Services
FruitBreedomics MAB Services is designed as a tool to efficiently transfer Marker Assisted Breeding related information to public and commercial Rosaceae breeding programs in Europe. It is a service offered on a commercial basis targeting FruitBreedomics stakeholder breeding programs. In a first phase (2014) it will be provided by a reduced number of service providers related to project partners (INRA, FEM and IRTA). The service will be provided based on information gathered within FruitBreedomics and other relevant external sources.
FruitBreedomics MAB Services will include:
- On site visits to breeding programs
- Explanation on the possibilities of using markers in breeding programs
- Detailed planning for using Marker Assisted Breeding in the breeding programs
- On site assistance for application of protocol for collecting samples
- Genotyping service
- On site discussion of the genotyping results and
- Assistance in the selection phase based on genotyping results.
The information that FruitBreedomics project will provide to the service providers will be: SNP markers for a list of breeding traits in apple and peach, detailed information on populations where the markers have been identified, a pipeline for MAB, a sampling protocol, a genotyping protocol, results of the pilot studies, and assistance by FruitBreedomics partners in interpreting this information.
A short presentation of each one of the first three service providers (Vegepolys, FEM and IRTA) is included. The short presentation includes a brief description of the service providers, their capacities, facilities and contact information. From FruitBreedomics, we are convinced that this initiative should help commercial apple and peach breeding programs in successfully implementing Marker Assisted Breeding and thus increasing the efficiency of the selection of new varieties.
IRTA- Marker Assisted Breeding Service
IRTA (Institute of Agrifood Research and Technology) is a public company of the Government of Catalonia, linked to the Department of Agriculture, Food and Rural Action (DAR), subject to private law, created in 1985. IRTA-Marker Assisted Breeding Service is integrated in the Genomics and Biotechnology program (GiB) located in CRAG, campus Universitat Autònoma Barcelona, Bellaterra (Barcelona, Spain). CRAG is a consortium that joins different research groups from IRTA, CSIC, UAB and UB working on plant and animal genomics and genetics with a powerful Genotyping Service (http://www.cragenomica.es/core-facilities/genotyping) containing a wide range of equipment for medium to high-throughput sample processing.
On this webpage a link can be found to “Custom Made Plant Genotyping”, where examples of our MAB Service are presented. They can be divided into five groups:
- Cultivar identification (germplasm characterization, genetic variability studies, pedigree and quality control),
- Hybrid purity testing (purity test of hybrid seed production, new hybrid and pedigree control),
- Major gene detection (genetic map construction, QTL analysis, association genetics),
- Marker assisted selection (various species: resistance genes; peach/nectarine: acidity: acid vs. sub-acid, fruit shape: round vs. flat, fruit type: peach vs. nectarine, flesh color: yellow vs. white and red vs. yellow/white,flesh texture: melting vs. non melting and freestonevs. clingstone, maturation period: short vs. long, flowering time: early vs. late, apricot: Resistance to sharka and Self-(in)compatibility, plum: Self-(in)compatibility),
- Special breeding applications (whole genome selection: introduction of exotic alleles, variety re-synthesis: the genetic reassembly of a specific variety that permits to vary certain traits of interest without losing the overall characteristics of that variety).
The IRTA MAB Service is dedicated to bring Science to Application, which means in our case focusing on oriented science mainly in fruit trees, strawberries, melon and vegetables. Oriented Science means developing and applying tools (molecular markers) for Marker Assisted Breeding (MAB) programs. This has created from the beginning a demand from part of the breeders to implement such tools in order to speed-up their breeding programs, as it is very costly to establish/maintain a R&D department including laboratories. To cope with this demand and at the same time to bridge the gap between Science and Application, IRTA created already in 1999 the MAB Service and since then we are expanding our collaborations with breeders (companies).
The collaborations that are offered include sample analysis in our laboratories, consulting service and regular meetings, in which the results are discussed and new strategies are being elaborated. Our team consists of researchers (e.g. M.J. Aranzana and P. Arús, both members of the FruitBreedomics Project and W. Howad) and technicians.
T. : +34692083816
Fondazione Edmund Mach
The Edmund Mach Foundation promotes and carries out research, scientific experiments, education and training activities as well as providing technical assistance and extensions services to companies. These activities are aimed at promoting cultural and socio-economic growth in the agricultural sector and at developing the forestry and agro-alimentary systems, with particular regard for the environment and the safeguard of the territory of the Trentino region. Since 2008 the Edmund Mach Foundation has continued to pursue the objectives and activities of the Istituto Agrario di San Michele all’Adige, founded in 1874.
The Research and Innovation Centre (C.R.I.) carries out studies and innovations in the fields of agriculture, nutrition, and environment, with the aim to generate new sharing knowledge and to contribute to economic growth, social development and the overall improvement of quality of life.
The Sequencing and Genotyping Platform takes part in the research and development activity of the CRI by offering support at the researchers activity and with the development of new methods of experimental investigation. The first purpose of the Platform is the development of new investigative methods, with the acquisition of new knowledge in the green biotechnology field and the continuous improvement of present and future instruments.
Marker assisted breeding (MAB) permits the selection of plants carrying alleles conferring superior phenotypes through the screening of markers tightly linked to those traits. In practice, it is possible to select plants at the seedling stage to enable the breeders to retain only germplasm conferring desirable traits from an early stage. Usually MAB involves the screening of hundreds or thousands of seedlings: the main challenge faced is thus developing a pipeline that begins with a “lab friendly” and unambiguous seedling configuration in greenhouse, which then allows the rapid collection of samples avoiding cross contamination, implements a fast, cost-effective, high-throughput method of DNA extraction to be used for a small number of PCRs, and utilizes robust, reliable, and easy to interpret molecular markers.
The Marker Assisted Breeding (MAB) service for master traits (as resistance, texture, storage) is based on three basic assumptions: the presence of a MAB consultant, the use of gold standard markers and a well optimized workflow. The Sequencing and Genotyping Platform offers a reliable MAB service based on his expertise in the molecular breeding programme carried out at the Edmund Mach Foundation.
Vegepolys is a plant cluster* established in 2005. The objective of Vegepolys is to promote the achievement of innovative projects through the collaboration of research laboratories and companies from the plant sector to increase their business and competitiveness.
The R&D lab of Vegepolys takes an active part in this objective. Its main purpose is to meet the needs of small or medium-sized companies which do not have sufficient means to make their in-house R & D. The lab teams are specialized on plant breeding, phytochemistry and plant pathology.
The plant breeding team aims to answer requests from companies wanting to establish or expand breeding activities without having all the appropriate resources to do so.
Our main services include:
1. Expertise on plant breeding.
2. Assessment and management of germplasm, breeding populations or cultivars by phenotyping or genotyping.
3. Plant selection by molecular tools.
Vegepolys has its own biotech lab and greenhouses. It also works on technical platforms shared with INRA-Angers, AgroCampus Ouest and the University of Angers such as Analysis of nucleic acids, Imagery, Phenotyping tools, Collection of Microorganisms and Phytochemical analyzes.
Vegepolys has a long experience in Marker Assisted Breeding particularly in horticultural plants. Our experience allows us to understand the difficulties in the MAB of cross pollinated plants as fruit trees. In any case we adapt our services to the needs and resources of companies and provide analysis and data interpretation while ensuring the confidentiality of the information.
Thanks to the knowledge and support researchers of Fruitbreedomics project, the laboratory of Vegepolys will very soon develop a "turnkey" service of MAB for fruit trees. Genetic markers serve as powerful breeding tools for quick and accurate selection and identification of plant specific traits.
Vegepolys is an association and any company can be member to benefit of networks, events and advices of the plant cluster. Moreover, members have discount on the service prices.
Visit our website: http://www.vegepolys.eu/en/
(*)The plant cluster is working especially on ornementals horticulture, viticulture, seeds, fruit & vegetables, medicinal and aromatic plants, mushrooms, apple cider.
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